A simple reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous estimation of atropine sulphate, chloramphenicol and dexamethasone sodium phosphate in eye drops formulation. The determination was carried Hiber® 250-4.6 mm, Lichrosher® 100, RP-18 (5 µm, 250 mm L × 4.6 mm Ø) column using a mobile phase of acetonitrile and phosphate buffer (pH 3, 0 min 10:90% v/v and 10 min 60:40% v/v, gradient). The flow rate was 1.2 ml/min with detection at 227 nm. The retention time for atropine sulphate was 9.34, for chloramphenicol 10.62 min and for dexamethasone sodium phosphate 13.50 min. The linearity range was 120-200 µg/ml, 60-100 µg/ml and 12-20 µg/ml for atropine sulphate, chloramphenicol and dexamethasone sodium phosphate respectively. The percentage recoveries obtained for atropine sulphate, chloramphenicol and dexamethasone sodium phosphate ranges from 99.62% - 100.62%, 99.23% - 98.70% and 98.62% - 100.73% respectively.
Loading....